The Clone Wars
"What a generous offer, Count. But our loyalty is reserved for only each other."
―Mother Talzin turning down Count Dooku's offer[src]

The Nightsisters were a clan of witches that lived on the mysterious world of Dathomir. As one of the many clans of witches on Dathomir, the Nightsisters chose to live apart from these other groups choosing to develop the malicious aspects of their mystic abilities. Originally founded by outcasts who had exiled from their respective clans. In time the Nightsisters grew into a powerful force in Dathomiri politics. Like all witches on Dathomir had a matriarchical society where men lived as servants, slaves and occasionally mates. This role in relation to the Nightsisters is filled by the Zabrak Nightbrothers.

Despite their power, the Nightsisters avoided the conflict of the Republic and Sith, content to pursue their own matters. The Jedi give them a wide berth, for they remain focused on matters on Dathomir. Count Dooku had dealings with them in the past, and turned to them when he was in need of a new apprentice.